PRODUCT CLASSIFICATION
產(chǎn)品分類Oxford Expression Technologies 重組蛋白
Oxford Expression Technologies公司簡介
Oxford Expression Technologies(英國)位于英國牛津,為全球制藥和生物技術行業(yè)提供產(chǎn)品,服務和咨詢服務。
Oxford Expression Technologies 簡稱(OET)專門從事重組蛋白表達,其核心技flashBACTM是一種基于桿狀病毒的蛋白表達系統(tǒng),能快速生產(chǎn)適用于小型和大型蛋白表達系統(tǒng)的多種重組病毒。
Oxford Expression Technologies簡稱(OET)產(chǎn)品還涉及昆蟲和哺乳動物表達系統(tǒng)中的載體、轉染試劑和培養(yǎng)基。
蛋白質印跡
該分析使用凝膠電泳分離的提取物中抗體與變性蛋白質的結合。它對于識別與其他蛋白質的脫靶結合非常有用。它還可以與二維分析相結合,二維分析在第一維通過電荷分離蛋白質,然后在第二維通過大小分離。這種更先進的方法可識別與預期蛋白質大小相同的非靶蛋白是否與抗體結合。
共聚焦顯微鏡
我們可以使用牛津布魯克斯大學(Oxford Brookes University)全guo最xian進的共聚焦顯微鏡設備之一。共聚焦顯微鏡是研究抗體在細胞內(nèi)非變性狀態(tài)下與其靶標相互作用的理想選擇。任何所需的細胞培養(yǎng)物都可以用重組桿狀病毒或陰性對照感染(昆蟲)或轉導(哺乳動物和禽)。然后用一抗處理細胞,然后用標記的二抗孵育,該二抗在適當波長的光下產(chǎn)生熒光。
酶聯(lián)免疫吸附法
這是抗體最常見的應用之一,特別是在診斷試劑盒中。至關重要的是,使用的任何抗體都可以被信任以結合正確的蛋白質靶標。在此類測試中,經(jīng)過驗證的陽性對照至關重要。雖然可以使用來自表達重組蛋白的細胞的粗提取物,但最好使用純化的材料。這也是提供陰性對照很重要的地方。我們可以模擬從這些樣品中提取蛋白質,并證明重組蛋白來源中沒有其他任何東西可以與靶抗體結合。
宿主細胞的選擇
大多數(shù)重組蛋白表達系統(tǒng)局限于狹窄范圍的宿主細胞。桿狀病毒系統(tǒng)的一個主要優(yōu)點是,它可用于在生產(chǎn)性病毒感染后在昆蟲細胞中制造蛋白質,或通過轉導在哺乳動物和禽類細胞培養(yǎng)物中制造蛋白質。由于蛋白質的翻譯后修飾可能是抗體結合的關鍵因素,因此使用來自細胞的材料來測試這一點是值得的,因為這些材料與預期靶標密切相關。
Oxford Expression Technologies公司的熱銷產(chǎn)品:
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
flashBAC | OET-100150 | 5 reactions |
flashBAC | OET-100151 | 24 reactions |
flashBAC | OET-100152 | 96 reactions |
flashBAC GOLD | OET-100200 | 3 reactions |
flashBAC GOLD | OET-100201 | 5 reactions |
flashBAC GOLD | OET-100202 | 24 reactions |
flashBAC GOLD | OET-100203 | 96 reactions |
flashBAC ULTRA | OET-100300 | 5 reactions |
flashBAC ULTRA | OET-100301 | 24 reactions |
flashBAC ULTRA | OET-100302 | 96 reactions |
flashBAC ULTRA | OET-100304 | 3 reactions |
flashBAC selection box 1 | OET-100400 | 3 x 3 reactions |
flashBAC selection box 2 | OET-100401 | 4 x 3 reactions |
flashBAC PRIME | OET-100500 | 5 reactions |
flashBAC PRIME | OET-100501 | 24 reactions |
baculoQUANT All-in-one virus extraction & titration kit | OET-100602 | 100 reactions |
titrePLUS flashBAC all-in-one | OET-100710 | 5 + 100 reactions |
titrePLUS flashBAC all-in-one | OET-100711 | 24 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100712 | 5 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100713 | 24 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100714 | 5 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100715 | 24 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100716 | 5 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100717 | 24 + 100 reactions |
BacPAK6 Linearised DNA | OET-101101 | 5 reactions |
BacPAK6 Linearised DNA | OET-101102 | 24 reactions |
BacPAK6 Linearised DNA | OET-101103 | 96 reactions |
BacPAK6 sec+ Linearised DNA | OET-101104 | 5 reactions |
BacPAK6 sec+ Linearised DNA | OET-101105 | 24 reactions |
BacPAK6 sec+ Linearised DNA | OET-101106 | 96 reaction kit |
pOET sequencing primers | OET-200100 | 2 x 100 ul |
pOET1 transfer plasmid | OET-200101 | 10 ug |
pOET1N_6xHis transfer plasmid | OET-2001011 | 10 ug |
pOET1C_6xHis transfer plasmid | OET-2001012 | 10 ug |
pOET2 transfer plasmid | OET-200103 | 10 ug |
pOET2N/C_6xHis transfer plasmid | OET-2001031 | 10 ug |
pOET2C_6xHis transfer plasmid | OET-2001032 | 10 ug |
pOET3 transfer plasmid | OET-200104 | 10 ug |
pOET4 transfer plasmid | OET-200105 | 10 ug |
pOET5 transfer plasmid | OET-200106 | 10 ug |
pOET6 BacMAM transfer plasmid | OET-200107 | 10 ug |
pOET1 Gateway® transfer plasmid | OET-200108 | 6 ug |
pOET6 BacMAM Gateway® transfer plasmid | OET-200109 | 6 ug |
pOET8.VE1 transfer plasmid | OET-200121 | 10 ug |
pOET8.VE2 transfer plasmid | OET-200122 | 10 ug |
pOET8.VE3 transfer plasmid | OET-200123 | 10 ug |
baculoFECTIN II | OET-300105 | 150 ul |
baculoFECTIN II | OET-300106 | 1ml |
baculoCOMPLETE protein expression kit | OET-400100 | 5 reactions |
baculoCOMPLETE protein expression kit + baculoQUANT all-in-one | OET-400101 | 5 + 100 reactions |
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傳真:86-010-62817090
地址:北京市昌平區(qū)城北街道鼓樓東街33號6號樓6428室